Abstract Detail

Abstract

Recently, multi-drug resistant (MDR) bacteria are responsible for a large number of infectious diseases that can be life-threatening. Globally, new approaches are targeted to solve this essential issue. This study aims to discover novel antibiotic alternatives by using the whole components of the biofilm layer as a macromolecule to synthesize silver nanoparticles (AgNPs) as a promising agent against MDR. In particular, the biosynthesized biofilm-AgNPs were characterized using UV-Vis spectroscopy, electron microscopes, Energy Dispersive X-ray (EDX), zeta sizer, and potential while their effect on bacterial strains, and normal cell lines was identified. Accordingly, biofilm-AgNPs have a lavender-colored solution, spherical shape, with a size range of 20–60 nm. Notably, they have inhibitory effects when used on various bacterial strains with concentrations ranging between 12.5 and 25 µg/mL. In addition, they have an effective synergistic effect when combined with phage ZCSE9 to inhibit and kill Salmonella enterica with a concentration of 3.1 µg/mL. In conclusion, this work presents a novel biosynthesis preparation of AgNPs using biofilm for antibacterial purposes to reduce the possible toxicity by reducing the MICs using phage ZCSE9.

Biography

Aghapy Yermans Yakoup is a graduate, batch 2023, with a biomedical sciences major (BMS) (medical sciences concentration) from Zewail City for Science, Technology, and Innovation. In addition, I have worked as a junior researcher assistant (jRA) in the Center for Microbiology and Phage Therapy (CMP) in Zewail City for Science, Technology, and Innovation from Fall 2021 until Summer 2023. I am working currently as an R&D specialist in Pharmaplast company. I am interested in finding new solutions to get rid of multi-drug-resistant bacteria and inventing new compounds that can be alternatives to antibiotics. Also, I am interested in the medical microbiology field. In the future, I am planning to enroll in a Ph.D. program that aims to find new applicable solutions for infectious diseases in different body systems like the nervous system and cardiovascular system.