Abstract Detail

Abstract

Background Infection is the second leading cause of death in patients with cancer. Loss of efficacy in antibiotics due to antibiotic resistance in bacteria is an urgent threat against the continuing success of cancer therapy. The advent of multidrug resistance among pathogenic bacteria is imperiling the worth of antibiotics, which have previously transformed medical sciences. The crisis of antimicrobial resistance has been ascribed to the misuse of these agents and due to unavailability of newer drugs attributable to exigent regulatory requirements and reduced financial inducements. Comprehensive efforts are needed to minimize the pace of resistance by studying emergent microorganisms, resistance mechanisms, and antimicrobial agents. 

Objective: Drug development from microbial endophytes of Talaromyces assiutensis and T. trachyspermus

Methods: In this study, different plant sample were collected from different areas of agriculture lands in order to isolate endophytic fungi. The isolated fungi were test for antibacterial activity against common laboratory bacteria and also checked their antifungal activity against root rot fungi. Gas Chromatography and Mass Spectrometry analysis of mycelial extract of Talaromyces assiutensis and T. trachyspermus were also performed in order to characterize compound from them as they showed strong antimicrobial activity and antifungal activity.   

Results:Endophytic fungi isolated from healthy plants have showed strong antibacterial activity against common laboratory bacteria (Salmonella typhimurium, Pseudomonas aeruginosa, and E.coli, Bacillus subtilis and Staphylococcus aureus) by forming the zone of inhibition. These endophytic fungi also showed strong potential against phytopathogens viz; Macrophomina phaseolina, Fusarium oxysporum, F.solani and Rhizoctonia solani. GC-MS spectroscopy of mycelial extract of Talaromyces assiutensis and T. trachyspermus revealed the presence of several compounds and some of them are new from this source as confirmed by computer matching against National Institute of Standards and Technology, USA (NIST Mass Spectrometry Data Center (mainlib) and finally compared with Science finder.

Conclusion: Antibiotic stewardship is, therefore, much needed to ensure not only the appropriate use of antimicrobials but also the choice of antimicrobials and their duration of use. The problem of antimicrobial overuse extends beyond antibiotic resistance and includes fungal and viral resistance.

Biography

I Dr Hafiza Farhat did Ph.D on Antibiotic producing endophytic fungi associated with healthy plants and received Ph.D degree from university of Karachi. Now I am working in well renowned university (Gomal University D.I Khan) of Pakistan as an Assistant Professor. I have research articles in high impact factor journal in national and international journals. I attended so many conference and workshop as a speaker.